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Data Collection for:

Carter et al. (2003). Journal of General Virology, 84, part 9, pp. 2443–2458.


Supplementary Data


The following material is available only in JGV Online:

Fig 1.   Genome analysis of recombinant viruses.

Virus genomes were analysed by PCR using oligonucleotides that flank the A5L or EGFP gene. PCR with primers flanking the A5L gene produced DNA fragments of 1.6, 2.3, 2.3, 1.6 and 1.6 kb for WT, vA5L-EGFP-C, vA5L-EGFP-N, vA5L-EGFP-C-rev and vA5L-EGFP-N-rev, respectively, whereas EGFP-specific primers produced a DNA fragment of 0.7 kb for vA5L-EGFP-C and vA5L-EGFP-N but not for WR or revertant viruses. The sizes of these DNA fragments confirmed that the chimaeric vA5L-EGFP-C and vA5L-EGFP-N gene was inserted into the A5L gene locus.




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