Supplementary figures
The p36 and p95 replicase proteins of Carnation Italian ringspot virus cooperate in stabilizing defective
interfering RNA, by V. Pantaleo, L. Rubino and M. Russo
Journal of General Virology vol. 85, part 8, pp. 2429-2433
Fig. S1. c-Myc-tagged p36 and HA-tagged p95 support DI RNA
replication. Upper panel, representative Northern blot of DI RNA
replicated by wild-type (lane 1) and tagged (lane 2) proteins. Lower
panel, mean relative accumulation and standard deviation of DI RNA as
determined for three independent experiments. Signals were measured with
Quantity One software (Bio-Rad).
Fig. S2. Mean relative accumulation and standard deviation of
proteins and DI RNA as in Fig. 3 in the paper, determined for three
independent transformants of each p36/p95 combination. DI RNA and protein
levels were normalized to those obtained with the highest or lowest copper
concentrations, respectively. Signals were quantified with Quantity One
software (Bio-Rad).