Vaccinia virus intracellular enveloped virions move to the cell periphery on microtubules in the absence of the A36R protein, by E. Herrero-Martínez, K. L. Roberts, M. Hollinshead and G. L. Smith
Journal of General Virology vol. 86, part 11, pp. 2961 - 2968
Supplementary Figures
Fig. S1. Genome analysis of recombinant viruses. (a) Virus genomes were analysed by PCR using oligonucleotides that flanked the A5L gene, amplified the EGFP gene or amplified the EGFPA5L chimeric gene, producing DNA fragments of 2.3, 0.7 and 2.0 kb for vDA36R-EGFPA5L, vDF12L-EGFPA5L and the vEGFPA5L positive control, respectively. The sizes of these DNA fragments confirmed that the chimeric EGFP gene had inserted into the A5L gene locus of vDA36R and vDF12L (see Methods for oligonucleotide sequences and a key to primer numbers). (b) Virus genomes were analysed by PCR using oligonucleotides that flanked the B5R gene, amplified the EGFPB5R chimeric gene or amplified the B5R SCR regions 2–4. The reactions produced DNA fragments of 1.7 and 1.6 kb for vDA36R-EGFPB5R and the vEGFPB5R positive control, respectively. A 0.5 kb SCR2–4 band was only found for the vEGFPA5L positive control that has a wild-type B5R gene. The sizes of these DNA fragments confirmed that the chimeric EGFPB5R gene had inserted into the B5R gene locus of vDA36R (see Methods for oligonucleotide sequences and a key to primer numbers).[ PDF file ] (679 KB)
Fig. S2. Time-lapse confocal movies corresponding to the cells shown in Fig. 5. Ptk2 cells infected with vB5REGFP (WR) [ Windows Media Audio/Video file ] (4.232 MB). Ptk2 cells infected with vΔA36R-B5REGFP (vΔA36R) [ Windows Media Audio/Video file ] (4.482 MB).
