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Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E

, by Nal, B., Chan, C., Kien, F., Siu, L., Tse, J., Chu, K., Kam, J., Staropoli, I., Crescenzo-Chaigne, B., Escriou, N., van der Werf, S., Yuen, K.-Y. and Altmeyer, R.

Journal of General Virology vol. 86, part 5, pp. 1423 - 1434

Supplementary figures [PDF] (829 KB)

Supplementary movie. Video sequence showing trafficking of vesicles containing SARS-M protein from and towards the Golgi apparatus. BHK-21 cells were grown on glass bottom microwell-petri dishes for 24 h prior to infection with recombinant SFV particles for M-EGFP expression. Images were taken every 10 s from 3.5 h post-infection. This film is made from a sequence of 105 snapshots (images 215 to 320) out of a total of 550 and displays six frames per second. [Movie] (5092 KB)







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