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evi
1
1 Crucell Holland BV, Archimedesweg 4, 2333 CN Leiden, The Netherlands
2 Ecole National Veterinaire d'Alfort, Alfort, France
3 Gaubius Laboratory, TNO Leiden, The Netherlands
4 Department of Endocrinology, Leiden University Medical Centre, Leiden, The Netherlands
Correspondence
Menzo Havenga
m.havenga{at}crucell.com
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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Published online ahead of print on 10 November 2005 as DOI 10.1099/vir.0.81293-0.
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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; Segerman et al., 2003; Sirena et al., 2004; Wu et al., 2004). Human subgroup B adenoviruses, Ad11 and Ad35, are rare in the human population resulting in low seroprevalence, whereas group C vectors (Ad2 and Ad5), which are commonly used for gene therapy and vaccination approaches, have high seroprevalence (Kostense et al., 2004; Nwanegbo et al., 2004; Vogels et al., 2003). High seroprevalence of Ad2 and Ad5 hampers the utility of these vaccine carriers since high anti-vector immunity effectively blunts vaccine potency (Barouch et al., 2004). Therefore, rAd11 and rAd35 vaccine carriers hold the promise of accurate dose control and consistent induction of immune responses against inserted antigens, which was further strengthened by a study showing strong rAd35-mediated induction of anti-SIVgag T-cell responses in naïve mice or in mice carrying high-level anti-Ad5 neutralizing activity (Barouch et al., 2004; Holterman et al., 2004; Vogels et al., 2003). Interestingly, this study also demonstrates that anti-Ad5 T-cells cross-reacting with rAd35, also known to be present in human PBMC samples (Heemskerk et al., 2003), only marginally affected the potency of the rAd35.SIVgag vaccine, indicating that neutralizing antibodies are predominantly responsible for the diminishing vaccine potency (Barouch et al., 2004). Thus far, studies with rAd11 or rAd35 vectors have been performed in inbred mice strains that do not express CD46 (Gaggar et al., 2003). In fact, whereas CD46 protein conservation is high between humans, new-world monkeys (80·2 %) and old-world monkeys (87·4 %), conservation is poor between humans and pigs, guinea pigs, rats and mice (all <50 %). Thus, we hypothesized that perhaps data generated so far on potency and distribution of rAd11 and rAd35 vectors in mice may not be representative of what can be expected of these vectors in monkeys and humans. Therefore, we set out to identify whether distribution and potency of rAd35 vectors differ in mice carrying the CD46 receptor or wild-type (Wt) littermates. Since the human CD46 protein (hCD46) serves as a high-affinity receptor for several important pathogens including Measles virus (Dorig et al., 1993; Manchester et al., 1994; Naniche et al., 1993), Human herpesvirus 6 (Santoro et al., 1999), Streptococcus pyogenes (Okada et al., 1995), Neisseria gonorrhoeae and Meningitides (Kallstrom et al., 1997), different CD46 transgenic mice models have been generated and studied (Horvat et al., 1996; Miyagawa et al., 1997; Rall et al., 1997; Thorley et al., 1997). In choosing among different CD46 transgenic mouse models, we decided on MYII mice, which were obtained by transferring a 420 kilobase yeast artificial chromosome clone carrying the hCD46 gene under its own regulatory sequences (Yannoutsos et al., 1996). These mice express all four major isoforms of CD46 known to be expressed in humans (Kemper et al., 2001; Yannoutsos et al., 1996), i.e. BC1, BC2, C1 and C2, and have been used successfully to establish animal models, for instance for measles virus infection (Oldstone et al., 1999). Based on the studies described here, showing MYII-specific lung sequestration of rAd35 upon intravenous (i.v.) administration, increased level and longevity of gene marking upon intramuscular (i.m.) administration in muscle and a clear tetramer-positive CD8+ T-cell dose-sparing effect upon rAd35.SIVgag vaccination, we conclude that MYII mice provide an interesting preclinical model for testing gene transfer and vaccination regimens involving rAd35 vectors.
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METHODS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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; Holterman et al., 2004; Vogels et al., 2003). The B16F10 mouse melanoma cell line was obtained from the ATCC and was stably transfected using lipofectamine, with a plasmid expressing the BC1 isoform under the control of the SV40 promoter. Transfectants were selected by co-transfecting a second plasmid, expressing neomycin under the control of the cytomegalovirus promoter, by adding 2·0 mg neomycin ml–1 to cell culture medium. High CD46-expressing cell clones were obtained via FACS sorting (Vantage SE; Becton Dickinson) and cell clone expansion upon limiting dilution. For vector transduction, cells were exposed for 2 h to 1000 or 2500 virus particles (vp) per cell after which virus containing medium was replaced by fresh medium. The percentage of cells expressing green fluorescent protein (GFP) was determined 48 h after vector exposure using FACSCalibur (Becton Dickinson). Human and rhesus monkey monocyte-derived dendritic cells (DC) as well as mouse bone marrow-derived DC of different strains were cultured and characterized essentially as described previously (Ophorst et al., 2004).
CD46 expression and haemagglutination.
To analyse CD46 surface expression by FACS, cells were stained for 30 min at 4 °C with 1 : 20 diluted (PBS/0·5 % BSA) anti-human CD46 coupled to fluorescein isothiocyanate (FITC; Leinco). For Western blot analysis, frozen tissue of human (n=1), monkey (n=1) or mouse origin (n=3) was homogenized with a blender in appropriate volumes of RIPA (0·5 g deoxycholic acid, 1 % NP40, 10 % SDS and PBS) buffer containing protease inhibitors (mini complete; Roche). Protein lysates were clarified by centrifugation and total protein content was determined using the Pierce BCA kit. Of each tissue, 10 µg total protein was loaded onto a 4–12 % Bis/Tris gel (Invitrogen) and transferred onto an Immobulon-P transfer membrane (Millipore). After blocking with TBST (0·1 % Tween 20 in Tris-buffered saline) containing 5 % (w/v) non-fat dry milk (Bio-Rad), membranes were incubated for 1 h with a 1 : 200 diluted goat polyclonal anti-CD46 antibody (Santa Cruz Biotechnology). Subsequently, a 1 : 15 000 diluted horseradish peroxidase-coupled rabbit anti-goat IgG polyclonal antibody (Santa Cruz Biotechnology) was used to visualize signals by enhanced chemiluminescence detection kit (ECL; Amersham Biosciences). For haemagglutination assays, mouse blood was diluted to 3 % erythrocyte density in preservation medium (Sanquin). Adenovirus stock solutions of rAd5 and rAd35 were diluted from 2x109 to 9·8x105 vp per 100 µl PBS containing 5 % sucrose, 0·7 mM CaCl2 and 0·7 mM MgCl2. As negative control only PBS containing 5 % sucrose, 0·7 mM CaCl2 and 0·7 mM MgCl2 was used. The 3 % erythrocyte solution was further diluted to 0·4 % erythrocyte density in PBS. Subsequently, 50 µl 0·4 % erythrocyte solution was added and plates were incubated for 1 h at 37 °C at 10 % CO2. Before read-out, plates were centrifuged for 1 min at 201 relative centrifugal force.
Breeding of MYII mice and Wt littermates.
For breeding purposes, Wt C57BL/6 mice were obtained through a registered breeding facility (Harlan, The Netherlands). MYII mice were obtained from Dr Grosveld (Erasmus University of Rotterdam, The Netherlands). Animal studies were performed according to Dutch law (Dutch Animal Experimentation Act) and the guidelines from the council of the European Committee (EU Dir. 86/609) on the protection of experimental animals. MYII mice and offspring were housed under quarantine conditions in individual ventilated type II cages (Techniplast), since their microbial status did not meet the FELASA standard. MYII mice and Wt littermates were obtained via breeding of MYII males with Wt C57BL/6 females after more than six rounds of backcrossing to C57BL/6. Genetic screening of offspring was performed through DNA analysis of tail tissue. Mouse DNA was purified using the Wizard SV 96 genomic DNA purification system (Promega). To discriminate between Wt littermates and MYII mice, oligonucleotides: MCPExon6F (5'-CAGTACTTG GGATCCCCCAGTTCC-3') and MCPExon7R (5'-GGTTTTGTACTAGATGGA GGCAGCA-3') were used in a PCR assay on purified mouse DNA. Male and female offspring between 6 and 12 weeks of age were used for all the mice experiments described. Experiments described here were all performed with mice housed under quarantine conditions given their health status, whereas MYII mice adhering to the FELASA standard have in the meantime been successfully obtained for future studies through embryo transfer at Harlan.
Distribution experiments.
Wt littermates and MYII mice (n=3 per group) were injected i.v. via the tail vein or i.m. via the musculus quadriceps with 1011 rAd5.Luc or rAd35.Luc vp in 50 µl PBS containing 5 % sucrose, 0·7 mM CaCl2 and 0·7 mM MgCl2. Transgene expression in these mice was assessed 24, 48 and 72 h after vector administration and directly after intraperitoneal (i.p.) injection of 2 mg per mouse luciferin in 30 µl PBS. A peltier cooled CCD camera system NightOWL (Berthold Technologies) coupled to the WinLight32 software was used for this purpose. For Q-PCR analysis, MYII mice and Wt littermates (n=10 per group) were injected intravenously (i.v.) with 1011 rAd35.Luc vp. Three untreated mice were included as controls. Mice were sacrificed after 48 h and organs were isolated. For Q-PCR analysis, DNA was isolated from organs using the DNeasy tissue kit (Qiagen). DNA was analysed for the presence of Ad35 genome using forward primer 5'-GTTCAGGGCCAGGTAGACTTTG-3' and reverse primer 5'-CGCGGAAATTCAGGTAAAAAAC-3', both primers recognizing sequences present within the cytomegalovirus promoter present in rAd35, and VIC-CCCATTACGTGGAGGTTTCGATTACCG-TAMRA was used as a probe. To determine the number of copies per cell, Q-PCR analysis was performed on the same samples using 18S rDNA sequences as targets for primers as described previously (Klein et al., 1999, 2000). Protein extracts derived from isolated organs were obtained as described previously (Havenga et al., 2002; Ophorst et al., 2004) and luciferase reporter gene expression levels were determined using the Promega luciferase detection system. For immunohistochemical analysis of lung tissue, mice were injected i.v. with 1011 vp of rAd35.lacZ (n=5 per group) and were sacrificed 48 h after vector administration. Lung tissue was fixed for 30 min in 4 % formaldehyde. After overnight staining with 1 mg X-Gal ml–1, lung tissue was again exposed to 4 % formaldehyde for 6 h. After dehydration, 5 µm slides were generated that were stained using a standard haematoxylin-phloxine-saffron protocol.
Vaccination and immunological read-out.
To vaccinate animals via different routes, all mice received a dose of 108 vp of rAd35.SIVgag (n=5 per group) in PBS containing 5 % sucrose, 0·7 mM CaCl2 and 0·7 mM MgCl2. For i.m. immunization, animals received 2x50 µl via the musculus quadriceps, 2x10 µl intranasally (i.n.), 50 µl for both intradermal (i.d.) and i.v. routes, and 100 µl for i.p., subcutaneous (sc) and oral (po) routes. Four weeks after vaccination presence of SIVgag-specific T cells was determined in spleen via interferon (IFN) 
ELISPOT, whereas SIVgag-specific antibodies were determined by ELISA as previously described (Barouch et al., 2004; Sprangers et al., 2003). Induction of anti-SIVgag CD8+ T cells present in blood was determined over time using AL11 tetramer staining as described previously (Barouch et al., 2004). Adenovirus neutralizing antibodies were determined using an assay previously published (Barouch et al., 2004; Sprangers et al., 2003). Subsequent i.m. vaccination experiments were performed with high doses (109 vp) or low doses (107 vp) injected in 2x50 µl PBS containing 5 % sucrose, 0·7 mM CaCl2 and 0·7 mM MgCl2.
DC transfer experiments.
Isolation and characterization of DC derived from bone marrow of both mice strains was performed as described previously (Ophorst et al., 2004). DC were transduced with 20 or 2000 vp per cell of rAd35.SIVgag for 2 h, after which cells were seeded in fresh medium for another 48 h. Subsequently, 5x105 DC, corresponding to 107 and 109 vp per mouse, were transferred to homotypic mice via i.m. (2x50 µl PBS) administration (n=5 per group). Immunological read-out for the presence of anti-SIVgag-specific T cells after injection of genetically modified DC in mice was performed as described above.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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; Havenga et al., 2001, 2002; Holterman et al., 2004; Vogels et al., 2003), we observed that the receptor for human type 35, at least, was not conserved between species. This observation is exemplified in Fig. 1(a), demonstrating that rAd35 vector efficiently transduces monocyte-derived DC from human and rhesus monkeys, while DC derived from diverse mouse strains are refractory to rAd35 transduction. With the knowledge that subgroup B viruses can utilize different cellular attachment molecules (Mei et al., 1998, 2002) including CD46 (Gaggar et al., 2003), we tested whether our rAd35 vector also utilizes CD46 as a receptor. Hereto, we generated mouse B16F10 melanoma cells that stably express the BC1 isoform of hCD46 on the membrane (Fig. 1b) and observed that rAd35-mediated gene transfer of GFP improved from 10 % GFP-positive cells in B16F10 cells towards >90 % in B16F10-hCD46 cells (Fig. 1c). Thus, hCD46 expression dramatically augments rAd35 vector cell transduction of these otherwise refractory mouse cells.
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(a), hCD46 could not be detected in Wt littermates, whereas MYII mice displayed expression in all major organs with most pronounced expression in the lungs and kidneys and least expression in muscle. Similar Western blot analysis on protein samples derived from organs of non-human primate or human origin (Fig. 2b) indicated that in man and monkey, expression of CD46 is also most pronounced in the lungs and kidneys and low in muscle. To identify whether the hCD46 present in MYII mice functions as rAd35 receptor, we exposed primary bone marrow-derived DC of MYII mice and Wt littermates to rAd35.GFP and rAd5.GFP as a control. The efficient infection of DC derived from MYII mice with rAd35.GFP (Fig. 2c), coupled to detection of hCD46 on the membrane of these cells (Fig. 2d), indicates that hCD46 functions as rAd35 receptor in these mice.
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).
Collectively, these data show that: (i) Wt inbred strains of mice do not express a high-affinity receptor for rAd35; (ii) hCD46 expressed in MYII mice functions as a high-affinity receptor for rAd35; (iii) the low hCD46 protein expression in muscle and the high level in the lungs and kidneys of MYII mice is similar to the hCD46 profile in non-human primates and human, in spite of the fact that MYII mice contain 10 hCD46 gene copies (Yannoutsos et al., 1996); and (iv) erythrocytes of MYII mice express hCD46 capable of binding to the rAd35 vector.
Vector distribution of rAd35 vector in hCD46-transgenic mice
We have previously reported that i.v. administration of rAd35.Luc vector did not result in vector accumulation in any organ of different Wt inbred mice strains as demonstrated by the lack of luciferase activity (Vogels et al., 2003). Here, we demonstrate that high dose, i.v. delivery of rAd35.Luc vector into MYII mice also does not result in high-level luciferase expression in this organ or any other organ (Fig. 3a). As expected, administration of rAd5.Luc vector resulted in clear luciferase activity in liver of either MYII mice or Wt littermates. Next, we performed Q-PCR analysis on major organs of MYII mice and Wt littermates 48 h after systemic administration of 1011 vp of rAd35.Luc vector. With the exception of lung tissue, none of the tested organs derived from MYII mice sequestered rAd35. In addition, none of the organs derived from Wt littermates sequestered rAd35 (Fig. 3b). In lungs of MYII mice, low copy numbers of rAd35 genomes were consistently detected, which correlated with low but clearly detectable luciferase expression upon rAd35.Luc gene transfer using a sensitive ex vivo luciferase detection assay (Fig. 3c, lung: P=0·02, ANOVA). A subsequent identical experiment using rAd35.lacZ showed the presence of lacZ-positive cells in lungs that appeared to be predominantly epithelial cells (Fig. 3d).
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). Together, the data demonstrate that (i) rAd35 sequesters in the lungs of MYII mice in contrast with Wt littermates upon i.v. administration; (ii) rAd35 does not sequester in the liver of MYII mice in contrast to rAd5; and (iii) upon high-dose i.m. administration a higher level of luciferase expression is obtained in MYII mice as compared with Wt littermates, resulting in prolonged gene expression in spite of the fact that these mice express low-level hCD46 in muscle.
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ELISPOT assay. As shown in Fig. 5(a), marked differences were observed in vaccination responses using different routes of immunization with rAd35 vector (Kruskal–Wallis test for differences between route of administration P<0·0001). Clearly, T-cell responses obtained via i.m., i.n. or i.d. administration proved highly consistent with little variation between animals. In contrast, i.v., sc or i.p. delivery resulted in substantial variation between animals, whereas oral administration (po) resulted in absence of T-cell response. The high variation between animals after i.v., i.p. and sc administration does not seem to reflect technical failure, since anti-Ad35 vector antibody responses (Fig. 5b) proved to be very well conserved within groups of animals, in particular upon i.v. and i.p. delivery. No significant differences could be observed between MYII mice and Wt littermates in anti-SIVgag T-cell responses for any of the vaccination routes tested using a vaccine dose of 108 vp (Wilcoxon test: P-values i.m. 0·46, i.p. 0·70, sc 0·25, i.n. 0·62, po 0·32, i.d. 0·92 and i.v. 0·45). Antibody responses against the SIV-gag protein were low using Ad35.SIVgag vaccine, irrespective of the route or mouse strain immunized (data not shown). To investigate the possibility of vaccine threshold effects in relation to presence or absence of the hCD46 receptor, MYII mice and Wt littermates were vaccinated with either a high (109 vp) or low (107 vp) rAd35.SIVgag vaccine dose. For this experiment animals were vaccinated using the i.m. route, based on the consistent anti-SIVgag T-cell responses obtained using the 108 vp vaccine dose (Fig. 5a, i.m.). As shown in Fig. 6(a), vaccination at 109 vp did not result in significant differences in anti-SIVgag T-cell responses between MYII mice and Wt littermates (mixed models with fixed effects, P=0·12). In contrast, strong SIV-gag-specific tetramer-positive CD8+ response (>4 %) could be detected in MYII mice starting 14 days after immunization using a 107 vp vaccine dose, whereas no tetramer-positive CD8+ T-cell response could be detected in Wt littermates (Fig. 6b, P<0·001). The tetramer-positive CD8+ responses detected correlated with day 28 IFN- ELISPOT results, which also demonstrated clearly detectable IFN- producing T-cells in all vaccinated MYII mice as compared with no T-cell response in 4 of 5 Wt littermates (P=0·07). At a vaccine dose of 109 vp no difference in ELISPOT results was observed (Fig. 6c, P=0·47). In an effort to link the observed differences in the induction of anti-SIVgag CD8+ responses directly to the presence of hCD46, DC derived from MYII mice or Wt littermates were isolated and exposed ex vivo to 2000 or 20 vp per cell of rAd35.SIVgag vector. Forty-eight hours after a 2 h vector exposure, 5x105 DC were administered i.m. to homotypic mice. As shown in Fig. 6(d), clear tetramer-positive CD8+ T-cell responses could be observed in both mice strains after infusion of 5x105 DC exposed for 2 h to 2000 vp per cell. In contrast, clear, albeit low, tetramer-positive responses were observed only in MYII mice after receiving 5x105 DC exposed for 2 h to 20 vp per cell (Fig. 6e, P=0·03 using mixed models for difference between Wt and Tg over time). Again, day 28 IFN- ELISPOT results correlated with CD8+ tetramer staining, demonstrating clear detection of IFN- producing T cells in MYII mice (Fig. 6f, P=0·01). At 109 vp per 5x105 DC, no difference in the T-cell response was observed between MYII mice and Wt littermates (P=0·46).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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), on cell lines and primary cells, is important to achieve efficient in vitro transduction with Ad5 vectors (Hutchin et al., 2000; Li et al., 1999; Nalbantoglu et al., 1999; Orlicky et al., 2001; Stockwin et al., 2002). Likewise, it has been reported, and confirmed here, that expression of CD46 on cells significantly augments in vitro gene transfer using group B vectors (Holterman et al., 2004; Vogels et al., 2003). By exploiting the natural diversity in receptor usage among adenoviruses (Havenga et al., 2002; Von Seggern et al., 1998, 2000), we have identified vectors that significantly increase levels of gene expression in DC and fibroblasts (Ophorst et al., 2004; Rea et al., 2001b), human bone marrow stroma cells (Havenga et al., 2002), haematopoeitic stem cells (Knaan-Shanzer et al., 2001), synoviocytes (Goossens et al., 2001) and smooth muscle cells (Havenga et al., 2001, 2002). Such vectors are in demand within the field of regenerative medicine (de Bruijn et al., 1999; Lamme et al., 1998, 2000; Moutsatsos et al., 2001; Riesle et al., 1998) and ex vivo DC-based vaccination approaches (Rea et al., 2001a). Thus, the in vitro correlation between cellular receptor expression and vector transduction efficiency is relatively clear.
In contrast, the in vivo need for a high-affinity receptor to obtain efficient gene transfer has not been completely elucidated. For instance, it has been suggested by some that viral receptors are not major determinants in adenoviral transduction of muscle (Cao et al., 2001), whereas others have shown that transduction of skeletal muscle in a human CAR transgenic mouse model (Tallone et al., 2001) resulted in an approximately 10-fold improved gene transfer as compared with Wt mice (reviewed by Thirion et al., 2002). Also, a clear discrepancy between in vivo rAd5 distribution and CAR expression in mice upon systemic delivery has been reported, demonstrating that anatomical barriers impact on vector distribution (Fechner et al., 1999). Such anatomical barriers challenge the concept of adenoviral vector targeting since any vector, be it of human or non-human origin (Holterman et al., 2004; Kremer et al., 2000; Vogels et al., 2003; Xiang et al., 2002; Zakhartchouk et al., 2003), will need to circumvent such anatomical hurdles.
We have recently shown that in spite of huge differences in the ability of a panel of different fibre-chimeric adenoviral vectors to infect primary mouse muscle cells, DC and fibroblasts in vitro, all these vectors elicited similar anti-insert-specific immune responses in mice upon i.m. vaccination (Mercier et al., 2004; Ophorst et al., 2004). Also, clear antigen-specific cellular and humoral immune responses are consistently obtained after i.m. immunization with rAd11 and rAd35 vectors in Wt inbred mouse strains lacking a high-affinity receptor (Barouch et al., 2004; Holterman et al., 2004; Vogels et al., 2003). Such results suggest that in the absence of a high-affinity receptor skeletal muscle, transduction occurs in mice.
Although we observed induction of immune responses in Wt inbred strains, the absence of a high-affinity receptor may result in an altered potency or distribution of these vectors. For these reasons, and knowing that both monkeys and humans widely express CD46, we wanted to investigate the role of the CD46 receptor in in vivo rAd35 vector transduction efficiency, rAd35 distribution and rAd35 vaccine potential. We chose the MYII strain, since these mice express all four major hCD46 isoforms (Kemper et al., 2001; Yannoutsos et al., 1996). The MYII mice are further known to contain 10 hCD46 gene copies in their genome and earlier studies using Northern blot analysis have demonstrated that hCD46 expression was similar between humans and MYII mice in the liver and lungs, whereas in the kidneys, heart and spleen higher levels of hCD46 mRNA were detected (Yannoutsos et al., 1996). Liver tropism was assessed by CCD camera imaging and revealed that rAd35 vector does not sequester in liver upon i.v. administration of either MYII mice or Wt littermates. These data corroborate our earlier data in Wt mice (Vogels et al., 2003) and also data of Seshidhar Reddy et al. (2003), who demonstrated that an E1B-containing Ad35 vector displays lack of mouse liver tropism. It should be taken into account that MYII mice, in contrast to humans, express hCD46 on their erythrocytes, which may lead to capture of rAd35 vector upon i.v. administration obscuring liver infection in these animals (Yannoutsos et al., 1996). However, the data are also in line with a recent report by Ni et al. (2005), showing that rAd5 vectors carrying fibres derived from Ad11 or Ad35 do not target the liver of baboons and do not show endothelial damage and inflammation typically observed with Ad5-based vectors. Thus, the strong liver tropism observed with rAd5 vector, known to cause dose-dependent hepato-toxicity limiting rAd5 dosing, can perhaps be circumvented using rAd35 vectors. Our Q-PCR analysis revealed that rAd35, when given at high dose, consistently accumulates at low rAd35 genome copy numbers in the lungs of MYII mice, correlating with expression detected in this organ. These data, as well as lack of liver infection in hCD46 transgenic mice, are in further agreement with a recent report showing that an rAd11 vector does not accumulate in the liver and can be detected for a short period of time (<72 h) in the lungs of hCD46 mice (Stone et al., 2005).
Upon i.m. administration of rAd35 higher initial marker gene expression was detected in MYII mice as compared with Wt littermates, which most likely translated in the prolonged expression observed in these MYII mice. The clear difference in level and longevity in luciferase expression observed in muscle did not correlate with the vaccination data, showing no difference at either 109 or 108 vp of rAd35.SIVgag dose in T-cell induction levels. However, a clear and significant tetramer-positive CD8+ T-cell response was observed in MYII mice at the low 107 vp rAd35 vaccine dose in contrast to no response in Wt littermates. We hypothesize that only limited antigen expression is already sufficient to trigger full blown immune responses and, thus, that a difference between MYII mice or Wt littermates could be observed only at lower dosing.
To provide further evidence that the presence of the high-affinity receptor for rAd35 vector contributes to the antigen-specific immune response, we genetically modified ex vivo cultured DC derived from Wt littermates and MYII mice and reinfused cells into naïve recipients. The observed difference in the anti-SIVgag-specific CD8+ T-cell response in MYII mice as compared with Wt littermates directly stems from the ex vivo modified DC, suggesting that DC derived from MYII mice were more susceptible to rAd35, resulting in higher SIVgag antigen expression and processing.
Future immunohistochemical analysis on muscle sections after direct i.m. immunization with rAd35 vector are needed to formally prove that the presence of CD46 in the muscle of MYII mice results in increased uptake of rAd35 vector by professional antigen presenting cells as compared with Wt littermates. However, this experiment demonstrates that DC from MYII mice are able to become infected with rAd35 vector, resulting in antigen expression and processing at sufficient level to induce in vivo anti-SIVgag CD8+ responses upon reinfusion into naïve host.
In summary, the data obtained have demonstrated that in MYII mice the rAd35 vector displays increased targeting and expression of transgenes in lungs after i.v. administration, increased level and longevity of local expression upon i.m. administration, and a significant dose-sparing effect in tetramer-positive CD8+ T cell induction as compared with Wt littermates. As such, we conclude that MYII mice provide an interesting preclinical model in studies involving rAd35 vectors.
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ACKNOWLEDGEMENTS |
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Received 28 June 2005;
accepted 25 October 2005.
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