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Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains, by P. P. C. Mertens, N. S. Maan, G. Prasad, A. R. Samuel, A. E. Shaw, A. C. Potgieter, S. J. Anthony and S. Maan

Journal of General Virology vol. 88, part 10, pp. 2811 – 2823

Supplementary Fig. S1. Electrophoretic analysis of cDNA products from Seg-2 of BTV-1 isolates using the newly designed type-specific primer pairs 1A₁, 1A₂ and 1A₃, and a vaccine-specific primer pair, 1V.

Supplementary Fig. S2. Electrophoretic analysis of cDNA products from Seg-2 of BTV-2 isolates using the newly designed type-specific primer pairs 2A, 2W₁, 2W₃ and 2W₄.

Supplementary Fig. S3. Electrophoretic analysis of cDNA products from Seg-2 of BTV-4 isolates using the primer pairs 4W₁, 4W₂, 4F and 4V.

Supplementary Fig. S4. Electrophoretic analysis of cDNA products from Seg-2 of BTV-8 isolates using the primer pairs 8W₂ and 8W₄.

Supplementary Fig. S5. Electrophoretic analysis of cDNA products from Seg-2 of isolates of BTV-9 using type-specific primer pairs 9A₁ and 9A₂ and primer pairs 9E₂, 9W₁ and 9W₂, specific to field or vaccine strains, respectively.

Supplementary Fig. S6. Electrophoretic analysis of cDNA products from Seg-2 of BTV-16 isolates using the newly designed type-specific primer pairs 16A, 16E₃ and 16E₄.

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