A chimeric plum pox virus spreads less effectively than and cannot compete in a mixed infection with its parental wild-type viruses, by C. Dietrich, Q. Al Abdallah, L. Lintl, A. Pietruszka and E. Maiss
Journal of General Virology vol. 88, part 10, pp. 2846 – 2851
Supplementary Fig. S1. Competition of PPV-NAT, PPV-NAT/SoC and PPV-SoC in different mixed infections during mechanical passaging. Specific PCR fragments of PPV-NAT (496 bp), PPV-NAT/SoC (547 bp) and PPV-SoC (751 bp) are given. Viruses have been detected by RT-PCR in firstly inoculated plants (lanes 1–3) and after two passages (lanes 4–6). Virus combinations were PPV-NAT+PPV-SoC (lanes 1, 4), PPV-NAT/SoC+PPV-SoC (lanes 2, 5) and PPV-NAT+PPV-NAT/SoC (lanes 3, 6). After two passages, PPV-NAT outcompeted PPV-SoC (lane 4) and PPV-NAT/SoC (lane 6). A minor fragment in lane 5 (asterisk) indicates reduced presence of PPV-NAT/SoC in co-infection with PPV-SoC. Fragments were separated by PAGE (5 % gel). Fragments of the marker indicate 500, 600, 700 and 800 bp.
