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Molecular characterization of a novel Ljungan virus (Parechovirus; Picornaviridae) reveals a fourth genotype and indications of ancestral recombination, by C. Tolf, M. Gullberg, E. S. Johansson, R. B. Tesh, B. Andersson and A. M. Lindberg

Journal of General Virology vol. 90, part 4, pp. 843 - 853

Supplementary method. Predictions of secondary structures of Ljungan virus (LV) capsid proteins.

Supplementary Table S1. Primers used for RT-PCR amplification and sequencing of the LV 64-7855 strain.

Supplementary Table S2. Capsid protein VP1 sequence identities between LV 64-7855 strain and previously characterized LV strains.

Supplementary Fig. S1. Comparison of terminal regions and predicted cleavage sites of the polyprotein sequence of the 64-7855 strain with those of previously published LV strains (i.e. M1146, 87-012, 174F and 145SL) and the HPeV prototype strain 1 (Harris).

Supplementary Fig. S2. Predicted secondary structure features of the LV capsid proteins, showing the sequence variation observed between American and Swedish strains.

Supplementary Fig. S3. Bootscan analysis of phylogenetic relationships between genome sequences of the 64-7855 strain (used as query sequence) and previously characterized LV strains (87-012, 174F, 145SL and M1146).

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