Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression, by M.-Y. Cha, D.-K. Ryu, H.-S. Jung, H.-E. Chang and W.-S. Ryu
Journal of General Virology vol. 90, part 4, pp. 978 - 986
Supplementary Methods.
Supplementary Fig. S1. HBx expressed from endogenous HBx promoter rescued viral genome replication. Cells were transfected with 1.2-mer-WT or 1.2-mer HBx-null (15 µg per 100 mm plate) in the presence or absence of EnhI/Xp-HBx* (15 µg per 100 mm plate).
Supplementary Fig. S2. Subcellular localization of HA-tagged HBx was also dependent on the expression level of HBx.
Supplementary Fig. S3. Biochemical fractionation of HBx.
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