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Cross-talk between Rac1 and Cdc42 GTPases regulates formation of filopodia required for dengue virus type-2 entry into HMEC-1 cells, by H. Zamudio-Meza, A. Castillo-Alvarez, C. González-Bonilla and I. Meza

Journal of General Virology vol. 90, part 12, pp. 2902 - 2911

Supplementary Video S1. The association between DENV-2 DiD and HMEC-1 expressing EGFP–actin. The sequence shows live cell activity during a 20 min sequence that corresponds to images in Fig. 3(b) of the main paper. The sequence is reproduced at 1 frame per min and each frame was captured with an exposure time of 2 s. [Video S1] (3.4 MB)

Supplementary Video S2. HMEC-1 expressing EGFP–actin. (a) HMEC-1 cells maintained in culture under non-stimulation conditions. (b) HMEC-1 expressing EGFP–actin, inoculated with DENV-2 and then transferred to 37 °C. The 20 min sequence shows live cell activity and is reproduced as for Supplementary Video S1. Selected frames are shown in Fig. 4(a, b) of the main paper. [Video S2] (2.4 MB)

Supplementary Videos S3–S5. HMEC-1 expressing EGFP–actin. In each video: (a) shows HMEC-1 expressing EGFP–actin inoculated with DENV-2; (b) shows cells pretreated with NSC23766 (video S3), Y-27632 (video S4) or blebbistatin (video S5) and inoculated with DENV-2. The sequences correspond to images in Fig. 4(b, c) (S3), Fig. 4(b, d) (S4) and Fig. 4(b, e) (S5) of the main paper.

[Video S3] (2.5 MB)

[Video S4] (2.7 MB)

[Video S5] (2.5 MB)

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This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef