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Fig. 3. Effect of mutations at the second, third and fourth positions of the PN motif on YFV replication. (ac) Analysis of RNA synthesis in BHK-21 J cells transfected with RNA of mutants at the second (a), second and third (b) and fourth (c) PN positions. The mutants tested are indicated above each lane. (df) Viral growth kinetics of the indicated YFV mutants. BHK cells were infected at an m.o.i. of 1; the medium of the infected cells was sampled at the indicated times post-infection. Titres were determined by plaque assays on Vero cells.
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