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Single Radial Immunodiffusion Test for Detecting Antibodies Against Surface Antigens of Intracellular and Extracellular Vaccinia Virus

E. Norrby

The Department of Virology, Karolinska Institutet, School of Medicine, Stockholm 1, Sweden

Antibodies to surface antigens of intracellular naked vaccinia virus (INV) and in limited studies extracellular enveloped virus (EEV) were determined by single radial immunodiffusion tests (SRDT) with immobilized virions in agarose gels. Antibodies to INV were demonstrable in rabbit hyperimmune sera (one to four visible zones), smallpox convalescent sera and sera from re-vaccinated individuals. A difference in specificity of antibodies reacting with INV and EEV was detectable by SRDT. The SRDT provides a simple, reproducible and specific test for detection of antibodies against vaccinia or variola virus, but the technique requires large quantities of virions.

^* Permanent address: Department of Microbiology, Medical College Dhanbad, Bihar, India.

To whom reprint requests should be addressed.

Received 24 August 1976; accepted 12 January 1977.