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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: vir.0.006544-0v1 90/4/987    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Joh, J. Articles by Ghim, S.-J. Search for Related Content PubMed PubMed Citation Articles by Joh, J. Articles by Ghim, S.-J. Agricola Articles by Joh, J. Articles by Ghim, S.-J.

Macaca fascicularis papillomavirus type 1 (MfPV-1): a beta-genus non-human primate PV causing rapidly progressive hand and foot papillomatosis

¹ The James Graham Brown Cancer Center, The University of Louisville;
² The Mannheimer Foundation;
³ The Albert Einstein College of Medicine and The Albert Einstein Cancer Center;
⁴ The Jackson Laboratory

⁵ E-mail: sjghim01{at}louisville.edu

Papillomaviruses (PVs) are a group of small, non-enveloped DNA viruses that cause mucosal or cutaneous neoplasia in a variety of animals. While most papillomas will regress spontaneously, papillomas may persist or undergo malignant transformation. Aggressive, persistent, and extensive warts were observed on the hands and feet of a Cynomolgus macaque (Macaca fascicularis). The presence of PV in the wart biopsies was identified by immunohistochemistry and PCR amplification of PV DNA. The genomic DNA of this PV was cloned, sequenced, and designated as Macaca fascicularis papillomavirus type 1 (MfPV-1). Its genome was 7588 base pairs (bps) in length and the organization of its putative open reading frames (ORFs) (E1, E2, E6, E7, L1, L2, and E4) was similar to other PVs. MfPV-1 has a short non-coding region (NCR) of 412 bps. Molecular analysis of MfPV-1 genomic DNA classified it into the beta-PV genus, to which all Epidermodysplasia verruciformis (EV)-type PVs belong. Diseases caused by PVs of the beta-genus are usually associated with natural or iatrogenic immunosuppression. The genomic characterization performed in this study shows that MfPV-1 clusters within the beta-PV genus and also contains EV-type specific motifs in its NCR. Further characterization of this virus and its host interactions may allow us to develop a non-human primate model for beta-genus HPVs, a genus populated by HPV types causing EV.

Received 22 August 2008; accepted 7 January 2009.