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This Article Full Text (Papers in Press[PDF]) Erratum All Versions of this Article: vir.0.006924-0v1 vir.0.006924-0v2 90/7/1592    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Knutson, B. A. Articles by Broyles, S. S. Search for Related Content PubMed PubMed Citation Articles by Knutson, B. A. Articles by Broyles, S. S. Agricola Articles by Knutson, B. A. Articles by Broyles, S. S.

Downregulation of vaccinia virus intermediate and late promoters by host transcription factor YY1

Purdue University

¹ E-mail: broyles{at}purdue.edu

Approximately half of vaccinia virus' intermediate and late gene transcriptional promoters have a binding site for the cellular transcription factor YY1 overlapping their initiator elements. Depletion of YY1 using RNA interference enhanced the activity of these promoters, while overexpression of YY1 repressed their activity. Viral promoter nucleotide replacements that specifically impair YY1's binding mostly alleviated the transcriptional repression, and correlated with the ability of YY1 to stably interact with the initiator DNAs in vitro. The transcriptional repression activity was localized to the C-terminal DNA binding domain of the protein. These results indicate that YY1 functions to negatively regulate these vaccinia virus promoters by binding to their initiator elements.

Received 28 August 2008; accepted 15 March 2009.