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Profile of physical status and gene variation of HPV 58 genome in cervical cancer

¹ Jilin University;
² Sichuan Tumor Hospital

³ E-mail: zha530909{at}sina.com

Epidemical studies showed that human papillomavirus 58(HPV 58) has a relatively high frequency in East Asia and some region of central and South America. To investigate the physical status of HPV 58 and analyze sequence variations of HPV58 in cervical cancer patients, the HPV 58 genome in 37 HPV 58 positive cervical cancer specimens collected form China were investigated by a mapping analyses based on nested PCR and nucleotide sequencing. In result, a pure integrated genome was found in 78.4 %( 29/37) specimens, which is much higher than those in previous studies. Multiple disruptions were first found among the integrated HPV 58 genomes in 51.7 %( 15/29) specimens. In 7,824 base pair nucleotide of HPV 58 genome, 119 (1.52%) nucleotide positions were found to be variable, and 45 of them lead to amino acid changes. Phylogenetic analyses based on partial L1 sequences of 14 variants isolated in previous studies and this study shows that two main groups were observed in HPV 58 variants, the prototype or prototype-like group and non-prototype-like group.

Received 24 October 2008; accepted 7 January 2009.