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Published online ahead of print on 4 March 2009 as doi:10.1099/vir.0.009274-0
Journal of General Virology 2009;90:1359.

A more recent version of this article appeared on June 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.009274-0
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The ORF3 protein of Hepatitis E virus interacts with the b beta chain of fibrinogen resulting in decreased fibrinogen secretion from HuH-7 cells

Ruchi Ratra, Anindita Kar-Roy and Sunil K. Lal1

ICGEB

1 E-mail: sunillal{at}icgeb.res.in

The ORF3 protein of Hepatitis E virus (HEV), the precise cellular functions of which remain obscure, was used in a yeast two-hybrid screen to identify its cellular binding partners. One of the identified interacting partners was fibrinogen B beta protein. The ORF3-fibrinogen B beta interaction was verified by co-immunoprecipitation and fluorescence resonance energy transfer in mammalian cells. Fibrinogen is a hepatic acute phase protein and serves as a central molecule that maintains host homeostasis and hemostasis during an acute phase response. Metabolic labeling of ORF3 transfected HuH-7 cells showed that the secreted as well as intracellular levels of fibrinogen were decreased in these cells compared to vector transfected controls. Northern hybridization and reverse transcription-PCR analyses revealed that the mRNA levels of all three chains of fibrinogen, A alpha, B beta and gamma, were transcriptionally down-regulated in ORF3 transfected cells. The constitutive expression of fibrinogen genes can be significantly up-regulated by IL-6, an important mediator of liver specific gene expression during an acute phase response. Transcription of fibrinogen genes after IL-6 stimulation was lesser in ORF3 expressing cells compared to controls. This report adds one more biological function to and advances our understanding of the cellular role of the ORF3 protein of HEV. The possible implications of these findings in the virus life cycle have been discussed.

Received 1 December 2008; accepted 19 February 2009.




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