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Published online ahead of print on 12 March 2009 as doi:10.1099/vir.0.009647-0
Journal of General Virology 2009;90:1767.

A more recent version of this article appeared on July 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.009647-0
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Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling

Khalid Amari1, Lorenzo Burgos1, Vicente Pallas2 and Maria Amelia Sanchez-Pina1,3

1 CEBAS-CSIC;
2 Instituto de Biologia Molecular y Celular de Plantas-CSIC

3 E-mail: spina{at}cebas.csic.es

The aim of this work was to follow infection by Prunus necrotic ringspot virus (PNRSV) in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the nucellus and, in some cases, in the embryo sac. Studies within infected seeds at the embryo globular stage revealed that PNRSV infects all parts of the seed including embryo, endosperm and testa. In the torpedo and bent cotyledon developmental stages, testa and endosperm high concentrations of the virus were detected. At seed maturity, PNRSV was accumulated slightly more in the embryo than in the cotyledons. In situ hybridization showed the presence of PNRSV RNAs in embryos obtained by hand-pollinating virus-free pistils with infected pollen. Interestingly, tissue-printing from fruits obtained from virus-free pistils hand-pollinated with infected pollen, showed viral RNA in the periphery of the fruits, whereas crosses between infected pistils and infected pollen resulted in a total invasion of the fruits. Taken together, these results shed light on the PNRSV vertical transmission from gametes to seedlings.

Received 16 December 2008; accepted 4 March 2009.




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