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Genetic and epidemiological characterisation of Stretch Lagoon orbivirus, a new orbivirus species isolated from Culex and Aedes mosquitoes in northern Australia

¹ CSIRO Livestock Industries;
² Center for Infection and Immunity, Mailman School of Public Health, Columbia University;
³ Berrimah Veterinary Laboratories

⁴ E-mail: chris.cowled{at}csiro.au

Stretch Lagoon orbivirus (SLOV) was isolated in 2002 from pooled Culex annulirostris mosquitoes collected at Stretch Lagoon, near the Wolfe Creek national park in the Kimberley region of Western Australia. Conventional serological tests were unable to identify the isolate and electron microscopy indicated a virus of the family Reoviridae, genus Orbivirus. A cDNA subtraction method was used to obtain approximately one third of the viral genome, and further sequencing was performed to complete the sequences of segment 1 (viral polymerase) and segment 2 (conserved inner core protein). Phylogenetic analysis shows that SLOV should be considered a new species within the genus Orbivirus. A real-time reverse transcription (RT)-polymerase chain reaction (PCR) test was designed to study the epidemiology of SLOV in the field. Six additional isolates of SLOV were identified, including isolates from four additional locations and two additional mosquito species. Horses, donkeys and goats were implicated as potential vertebrate hosts in a serological survey.

Received 4 January 2009; accepted 8 March 2009.