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Full-length infectious clone of Beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves

¹ Université catholique de Louvain (UCL);
² Institut de biologie moléculaire des plantes, Université Louis Pasteur, Strasbourg

³ E-mail: francois.crutzen{at}uclouvain.be

For a better understanding of the functionality and pathogenicity of Beet soil-borne virus (BSBV), full-length cDNA clones have been constructed for the three genomic RNAs . With the aim of assessing their effectiveness and relative contribution to the virus house-keeping functions, transcripts were inoculated on Chenopodium quinoa and Beta macrocarpa leaves using five genome combinations. Both RNAs-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication in planta and symptom production on C. quinoa, whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not. No symptoms were recorded on B. macrocarpa, but viral RNAs were detected. In both host plants, the 19 kDa CP was detected by Western blotting as well as a 115 kDa protein corresponding to the CP-RT.

Received 23 June 2009; accepted 1 September 2009.