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Front cover illustration
Localization of MP-red fluorescent protein and tubulin-GFP in epidermal cells. Genes encoding the wild-type (top panel) 3a protein, as well as the MPs of the dysfunctional mutant M4 (middle panel) and the conditionally functional mutant M8 (bottom panel), were cloned in the binary vector pROK2 and fused at their C termini to the monomeric red fluorescent protein (mRFP). These binary constructs were agroinfiltrated into either transgenic Nicotiana benthamiana plants expressing α-tubulin fused to GFP (tua-GFP) for transient expression of the tagged MPs (wild-type and M4), or non-transgenic N. benthamiana co-infiltrated with a binary construct expressing tua-GFP (M8). Image courtesy Professor Peter F. Palukaitis, Scottish Crop Research Institute, Invergowrie. See the paper by Canto & Palukaitis in this issue, pp. 1223-1228.
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