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Front cover illustration

Visualization of prion dissemination in MovS co-cultures. MovS cultures were fixed, treated with guanidine thiocyanate to expose epitopes of abnormal PrP and labelled with ICSM33 anti-PrP mAb. Alexa-conjugated IgG was used as the secondary antibody. Nuclei were stained with DAPI. Infected MovS cells diluted 100-fold with permissive MovS cells were co-cultivated for 5 days in liquid culture medium (top) or in semi-solid medium (bottom). Image courtesy Dr Didier Vilette, UMR INRA, Toulouse, France. See the paper by Paquet et al. in this issue, pp. 706-713.