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Cover Figure


Front cover illustration

Indirect immunofluorescence analysis of a truncated NS4B protein of hepatitis C virus. An NS4B construct (NS4Bt110-260) was transiently transfected into Huh7 cells. After 48 h, cells were labelled with anti-Myc or anti-PDI antibody. NS4B proteins were immunostained with Alexa Fluor 488-labelled goat anti-mouse IgG (green), whilst PDI was stained with Alexa Fluor 568-labelled goat anti-rabbit IgG (red). DAPI staining (blue) revealed the nuclear chromatin. The bottom square shows the three images superimposed. Image courtesy N. Sakamoto, Tokyo Medical and Dental University, Tokyo, Japan. See the paper by Tasaka et al. in the December 2007 issue, vol. 88, part 12, 3323–3333.



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